Microwave-Assisted Enzymatic Preparation of Acylated Naringin in Non-aqueous and Its Anti-Proliferation Effect on HepG2 Cells
1. College of Life Science, Jilin University, Changchun 130023, China;
2. Key laboratory for Molecular Enzymology & Engineering the Ministry of Education, Jilin University, Changchun 130023, China
Abstract：Acetylated naringin was synthesized by microwave-assisted non-aqueous enzymatic reaction to improve the stability and physiological bioactivity. The effects of various parameters on the enzyme activity and anti-proliferation effect on HepG2 were investigated and optimized. The results showed that the enzyme activity reached (612±10) μmol(/h·g) under the optimum conditions of microwave power 400 W, microwave temperature 70 ℃ and water activity 0.80, which was about 4 times higher compared to traditional reaction. The maximum conversion was (90.42±2.43)% after reaction 2 h. The product was identified as 6’-acetylnaringin by nuclear magnetic resonance (NMR), the mass fraction was larger than 99%. Although the antioxidant activity of acetylated naringin in vitro was slightly lower than that of maternal naringin, it had a strong antiproliferative activity against HepG2 cells. That meant that the non-aqueous enzymatic acetylation of naringin has biological significance.
JIANG Liyan1,ZHAO Bo1,WANG Zhi2 et al. Microwave-Assisted Enzymatic Preparation of Acylated Naringin in Non-aqueous and Its Anti-Proliferation Effect on HepG2 Cells[J]. 化学反应工程与工艺, 2020, 36(1): 90-96.
