Abstract：The recombinant Eco(pETDuet-ATA117) was constructed for the functional expression of transaminase ATA117. After that, the response surface methodology was employed to optimize the fermentation conditions. A two-level fractional factor design was used to evaluate the effects of different components of medium. The results showed that glycerin, yeast extract and tryptone had important effects on transaminase activity, then the optimal response surface was determined by the Steepest ascent design, finally, the optimal medium composition was observed by Box-Behnken design: glycerin14.45 g/L, yeast extract 7.27 g/L and tryptone 5.72 g/L. Under the optimal conditions, the Enzyme activity(391.7 U/L) was much higher than that using either basal fermentation medium(125.4 U/L) or single variable optimization of fermentation medium(320.7 U/L). Meanwhile, the inducing conditions was also studied, and under the optimal induction conditions of 24 ℃, optical densityof 0.8 under 600 nm, the final Enzyme activity was 713.7 U/L after 15 h induction time when isopropyl thiogalactoside was 1 mmol/L.
Wang Lu,Xu Gang,Wu Jianping et al. The Expression of Recombinant Transaminase in Escherichia Coli BL21(DE3) and Optimization of the Fermentation Conditions[J]. 化学反应工程与工艺, 2014, 30(6): 513-521.
