The Process Development of (R)-1-Phenylethanol Asymmetric Reduction Catalyzed by A Recombinant Short-Chain Dehydrogenase and Reductase LcSDR
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
Abstract A short-chain dehydrogenases and reductases (LcSDR) coding sequence lcsdr was screened out from Lactobacillus composti genome via gene mining technology. It was found that LcSDR was able to asymmetrically reduce acetophenone(1a) to (R)-1-phenylethanol [(R)-1b]. The LcSDR and glucose dehydrogenase (EsGDH) double-enzyme conjugate catalytic synthesis system was constructed, and the process parameters were optimized for catalytic reduction of 1a to (R)-1b in this study. Under the appropriate conditions, 50 g/L 1a was converted to (R)-1b in 2 h with yield of 93.8%, the enantiomeric excess (eep) above 99% and the space time yield of 562.8 g/(L?d).
Chen Min,Wang Yajun,Liu Zhiqiang et al. The Process Development of (R)-1-Phenylethanol Asymmetric Reduction Catalyzed by A Recombinant Short-Chain Dehydrogenase and Reductase LcSDR[J]. 化学反应工程与工艺, 2016, 32(4): 320-325.
Chen Min,Wang Yajun,Liu Zhiqiang et al. The Process Development of (R)-1-Phenylethanol Asymmetric Reduction Catalyzed by A Recombinant Short-Chain Dehydrogenase and Reductase LcSDR[J]. 化学反应工程与工艺, 2016, 32(4): 320-325.
